Staff / Senior Scientist, Assay Development (Chemistry)

Job description

### **About Glyphic:** At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised >$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing. **What we are looking for in you** We are seeking a Senior or Staff Scientist to join our Assay Development team at a critical stage in platform development. Reporting to the Director of Assay Development, you will be a hands-on expert working at the chemistry-assay interface — developing the chemical methods that enable reliable, high-efficiency sample loading into our expansion assay, and building the novel conjugation, cleavage, and stabilization workflows that power our single-molecule proteome sequencing platform. You will work closely with the Chemistry team to develop sample input methods for new protein and peptide types, including side-chain protection strategies, encoder loading routes, and peptide-from-protein handoff approaches. The ideal candidate brings deep expertise in peptide chemistry, bioorthogonal and click conjugation strategies, and cleavage chemistry development — with a track record of building new chemical methods for complex, multi-component systems. You combine a synthetic chemist's mechanistic rigor with sensitivity to how chemistry performs inside a demanding multi-cycle assay environment. This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location. **What you’ll do** Expansion assay chemistry & sample loading - Optimize bioconjugation methods for peptide loading into the expansion assay; establish robust, high-efficiency loading protocols that perform reliably across diverse peptide populations and physicochemical characteristics. - Identify, characterize, and optimize peptide labeling during the ProSE sequencing assay, leveraging mechanistic understanding of Edman chemistry and amino acid side-chain reactivity and stability. - Develop reagents and methods to maximize the stability of peptides, DNA, and peptide-DNA chimeras throughout the ProSE sequencing assay. - Partner with the Chemistry team to develop amino acid side-chain protection chemistries. - Evaluate compatibility of new chemistries with existing assay components, reagents, and multi-cycle sequencing conditions. - Evaluate emerging chemistries from the literature and internal pipeline; synthesize and test candidate reagents. Novel assay chemistry workflows - Design and determine the order of operations for chemical manipulations within the assay workflow — including decisions on when specific steps occur on-bead versus off-bead, and how side-chain protection and deprotection strategies are sequenced relative to assay cycles — to ensure compatibility, efficiency, and analytical interpretability. - Optimize selective cleavage chemistry workflows for use within the sequencing assay. - Optimize for efficiency, selectivity, and compatibility with assay conditions. - Develop stabilization strategies for sensitive assay intermediates and conjugates. - Employ analytical methods — HPLC (RP and IEX), LC-MS/MS, UV-Vis, CE — to characterize conjugation efficiency, adduct identity, purity, and stability of assay intermediates and conjugates. - Characterize and troubleshoot the impact of the chemical steps of multi-cycle workflows. **What you need** Required: - Advanced degree in organic chemistry, chemical biology, biochemistry, or a related field - Industry experience designing multi-step chemical workflows in complex biological systems, including sequencing or determining the order of chemical manipulations across assay conditions. - MS/PhD with 6+/4+ years of experience (for Senior Scientist) - MS/PhD with 8+/6+ years of experience (for Staff Scientist) - Demonstrated experience developing site-selective bioconjugation chemistry for native peptides — including N-terminal, C-terminal, and side-chain modification strategies — using click chemistry (CuAAC, SPAAC, IEDDA), NHS-ester coupling, and related bioorthogonal approaches in multi-component peptide systems. - Experience developing multi-step workflows using peptides, oligonucleotides and bioconjugation techniques including in solution and on surfaces. - Proficiency with analytical characterization of complex conjugates and assay intermediates: HPLC (RP and IEX), LC-MS/MS, UV-Vis; demonstrated ability to develop and validate analytical methods for novel chemical entities. - Method-development mindset: history of building and adapting new chemical approaches rather than executing established protocols. - Ability to function as both an independent contributor and a collaborative partner in a cross-functional team environment. - Excellent problem-solving skills and attention to detail. - Excellent written and verbal communication skills, with the ability to work seamlessly with colleagues from diverse scientific and technical backgrounds. - Ability to thrive in a fast-paced environment with evolving priorities. Nice to have: - Familiarity with nanopore or single-molecule sequencing chemistry, and the demands it places on assay reagent purity, stability, and uniformity. - Experience with solid-phase peptide synthesis (SPPS), including on-resin modification, selective sidechain deprotection, and non-standard protecting group strategies. - Familiarity with statistical and DOE-based experimental design for chemistry optimization. - Prior experience in an early-stage biotech or tools/diagnostics company. We’re looking for a teammate that: - Navigates complex team dynamics, partnerships, and challenges with creativity and logic. - Operates with adaptability, urgency, and flexibility in evolving environments, thriving in ambiguity. - Drives work forward without needing to be asked, taking responsibility for outcomes rather than tasks. - Treats obstacles as problems to be creatively solved, not reasons something can’t be done. - Applies sound judgment to the best available information, testing, learning, and iterating. - Shares early and directly when assumptions change, results are unclear, or timelines are at risk. **What you can expect from this role** Work environment: - Collaborative culture where your ideas and expertise are valued - Direct impact on product development and company direction Professional growth: - Learn from a diverse team of world-class scientists and engineers - Contribute to first-of-their kind technologies, high-impact publications, and patents Compensation Estimated Base Salary $123,000 - $187,575 This is the pay range for this position that we reasonably expect to pay. Individual compensation is based on various factors including, experience, education, skillset, and geographic location. This range is for the SF Bay Area, California location and may be adjusted to the labor market in other geographic areas. ### Benefits and Perks: - Employee Stock Option Plan - 100% Health Plan Coverage for Employees & Dependents (Medical, Dental, & Vision) - Employer Retirement Contributions to 401(k) - Generous Paid Time Off - Paid Maternity and Paternity Leave - Health & Wellbeing Program - Office Snacks and Beverages - Regular Team Bonding Activities We are an Equal Opportunity Employer. We celebrate diversity and are committed to creating an inclusive environment for all employees. Individuals seeking employment at Glyphic Biotechnologies are considered without regard to race, color, religion, national origin, age, sex, marital status, ancestry, physical or mental disability, veteran status, gender identity, or sexual orientation.